Introduction – UVP Benchtop UV Transilluminators User Manual

Page 2

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Benchtop UV Transilluminators

Page 2

Introduction

The UVP Transilluminator offers the researcher uniform and intense sources of ultraviolet light
(radiation) in a compact package. The special design emits high intensity excitation UV wavelength
for back-illumination of transparent fluorescent materials. The 302nm UV back-illumination provides
a highly sensitive method to detect double-stranded nucleic acids that have been labeled with
fluorescent dyes such as ethidium bromide or acridine orange. Single stranded nucleic acids may
be detected, but with a lower excitation wavelength more sensitive for nucleic acid visualization
than the 365nm model.

The transilluminator is uniquely designed with increased UV intensity and uniformity, instant on
capabilities, no lamp flicker and reduced electrical consumption.

NOTE: Though the manual refers to the midrange UV waveband as 302nm, others refer to this
region as 300nm or 312nm. The spectral output of all these regions is the same.

A Word of Caution: UV Transilluminators are powerful sources of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area
are properly protected. If not using the transilluminator with an imaging system darkroom, a UV
Blocking Cover should be attached to the transilluminator. Even though this cover blocks the
ultraviolet radiation emitted by the unit, UV Blocking Eyewear should be worn as well.

UVP UV Transilluminator features:

UV Blocking Cover may be
included with the
transilluminator. The cover
is hinged and adjustable to
varying heights

Select models include a
control knob for selection of
wavelengths or intensity

Power switch

UVP’s long life filters are
available in a variety of
filter sizes from 15 x
15cm to 25 x 26cm

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