Bio-Rad PCR Plastic Accessories - Manual

Frame-Seal
™
Incubation Chambers
for Sealing Reactions on Slides
Frame-Seal incubation chambers provide vapor-tight sealing for FISH, Polony, in situ PCR,
and PRINS applications. The adhesive frames are placed over the specimen area and
fastened to the slide. Reaction mix is then added and sealed in place with a flexible plastic
coverslip. The gas-tight seal withstands temperatures up to 97°C. After cycling, the entire
chamber can easily be removed from the slide, and the reaction mix can be retrieved.
Frame-Seal chambers can be UV-treated and autoclaved (with a weight on top to keep
them flat), and are compatible with MJ-line and other horizontal-format slide thermal cyclers.
Directions for Use
1. Each frame comes sandwiched between two plastic liners — one thin rectangular liner
and one thicker liner with a rectangular hole in it. Lined frames are packaged as strips
with perforations between frames. Separate one frame from a strip and remove the
protective plastic liner that has the rectangular hole, leaving the frame adhered to the
thinner liner (Fig. 1).
2. Position the frame adhesive side down over the desired area of the slide (Fig. 2).
3. Remove the top liner (Fig. 3).
4. Pipet the appropriate volume of reagent into the frame at one end (Fig. 4).
5. The polyester covers are packaged in a separate bag. Remove one cover from the bag
and roll it over the frame, beginning at the end where the reagent was added (Fig. 5).
6. Rub all four edges with a blunt, hard object, such as a pen barrel, to ensure good
adhesion. The assembly is now ready to use.
Frame-Seal Chambers 100 frames and coverslips per package
SLF-0201 9 x 9 mm chambers, 25 µl capacity
SLF-0601 15 x 15 mm chambers, 65 µl capacity
SLF-1201 17 x 28 mm chambers, 125 µl capacity
SLF-3001 19 x 60 mm chambers, 300 µl capacity