Bio-Rad Genes in a Bottle™ Kit - Manual

1. Obtain 15 ml tube containing 3 ml water from your
instructor. Label the tube with your initials.
2. Gently chew the insides of your cheeks for 30 seconds.
It is NOT helpful to draw blood!
3. Take the water from the 15 ml tube into your mouth,
and swish the water around vigorously for 30 seconds.
4. Carefully expel the liquid back into the 15 ml tube.
5. Obtain the tube of lysis buffer from your workstation,
and add 2 ml of lysis buffer to your tube.
6. Place the cap on the tube, and gently invert the tube
5 times (don’t shake your tube!). Observe your tube
— do you notice any changes? If you do, write them
down.
7. Obtain the tube of protease (prot) at your workstation.
Add 5 drops of protease to your tube.
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Quick Guide for DNA Extraction and Precipitation