Document Outline

• EG – declaration of conformity
• Product registration
• 1. Safety
  • 1.1 Legal considerations
  • 1.2 Structure of the safety instructions
    • 1.2.1 Signal word panel
    • 1.2.2 Safety alert symbol
    • 1.2.3 Pictograms
    • 1.2.4 Word message panel structure
  • 1.3 Localization / position of safety labels on the unit
  • 1.4 Type plate
  • 1.5 General safety instructions on installing and operating the CO2 incubator
  • 1.6 Precautions when working with gases
  • 1.7 Precautions when handling gas cylinders
  • 1.8 Intended use
• 2. Unit description
  • 2.1 Unit overview
  • 2.2 Instrument panel
  • 2.3 Inner chamber
  • 2.4 Control panel on the rear of the unit
• 3. Completeness of delivery, transportation, storage, and installation
  • 3.1 Unpacking, and checking equipment and completeness of delivery
  • 3.2 Guidelines for safe lifting and transportation
  • 3.3 Storage
  • 3.4 Location of installation and ambient conditions
• 4. Installation and connections
  • 4.1 Shelves
  • 4.2 Permadry™ water pan
  • 4.3 Gas connections
    • 4.3.1 Connection of the CO2 gas cylinder
    • 4.3.2 Connection of the O2 gas cylinder (unit with O2 control)
    • 4.3.3 Connection of the N2 gas cylinder (unit with O2 control)
    • 4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2)
    • 4.3.5 Gas cylinder connection kits (option)
  • 4.4 Electrical connection
  • 4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment)
• 5. Start up
  • 5.1 Equilibration time
  • 5.2 Factory settings
• 6. Functional overview of the T4.12 chamber controller
  • 6.1 Menu structure
    • 6.1.1 General menu
    • 6.1.2 Quick menu
    • 6.1.3 User menu
  • 6.2 Operating modes
    • 6.2.1 Activating the “control off” mode or change to “fixed value” operating mode
  • 6.3 Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control)
    • 6.3.1 Required gas supply of the unit with O2 control
    • 6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms
  • 6.4 Humidity control of the Permadry™ system
  • 6.5 Turning on / off the interior socket voltage (with optional interior socket)
  • 6.6 Performance during and after power failure
  • 6.7 Information
• 7. Setpoint entry
  • 7.1 Setting ranges
  • 7.2 Note when setting high gas concentrations
  • 7.3 Entering the setpoints via “quick menu”
  • 7.4 Entering the setpoints via general menu
• 8. Key lock
  • 8.1 Directly activating the key lock
  • 8.2 Automatic key lock
  • 8.3 Changing the password for unlocking the key lock
• 9. General controller settings
  • 9.1 Setup wizard
  • 9.2 Date and time settings
  • 9.3 Selecting the menu language of the T4.12 controller
  • 9.4 Setting display brightness
  • 9.5 Changing the temperature unit
  • 9.6 Defining the data recording rate
  • 9.7 Factory reset
  • 9.8 Network configuration
  • 9.9 Display of the network configuration
  • 9.10 MAC Address
  • 9.11 RS 422 address (with optional RS 422 interface)
  • 9.12 Display and entry of the device configuration – for service purpose
• 10. Data transfer via USB interface
  • 10.1 Exporting data to USB drive
  • 10.2 Importing data from USB drive
• 11. Notifications and Alarms
  • 11.1 Notifications overview
  • 11.2 Alarm messages overview
  • 11.3 Alarm status
  • 11.4 Confirming an active “set” alarm
  • 11.5 Alarm configuration and overview
    • 11.5.1 List of active alarms
    • 11.5.2 History – list of all alarms
    • 11.5.3 Activating, deactivating, and testing the alarm buzzer
    • 11.5.4 Activating / deactivating all alarm functions
    • 11.5.5 Setting the delay time after opening the door
    • 11.5.6 Tolerance ranges and alarm delay times
  • 11.6 Zero-voltage relay alarm output
• 12. Event list
• 13. Graphical display of the measured values
  • 13.1 Setting the sampling rate
  • 13.2 Defining the display range
  • 13.3 Selecting the parameters
• 14. Temperature safety devices
  • 14.1 Overtemperature protective device (class 1)
  • 14.2 Overtemperature safety controller (temperature safety device class 3.1)
    • 14.2.1 Safety controller modes
    • 14.2.2 Setting the safety controller
• 15. Options
  • 15.1 Communication software APT-COM™ 3 DataControlSystem (option)
  • 15.2 RS 422 interface (option)
  • 15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)
  • 15.4 Interior socket 230V (option)
  • 15.5 Analog outputs for temperature and CO2 (option)
  • 15.6 Access port for extra-low voltage (option)
  • 15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option)
  • 15.8 Stands
    • 15.8.1 Stacking stand (option)
    • 15.8.2 Stacking adapter for direct thermal decoupled stacking (option)
    • 15.8.3 Base on castors (option)
• 16. Reference measurements
  • 16.1 CO2 reference measuring
    • 16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium
    • 16.1.2 Measuring CO2 directly via chemical indicator tubes
    • 16.1.3 Measuring CO2 directly with an electronic infrared measuring device
  • 16.2 Temperature reference measurement
• 17. Avoiding microbial contamination
  • 17.1 Cells and media
  • 17.2 Laboratory conditions / equipment around the incubator
  • 17.3 Working and behavior in the lab
  • 17.4 Chamber design and equipment of the CO2 incubator
  • 17.5 Handling the CO2 incubator
• 18. Cleaning, decontamination / disinfection, and sterilization
  • 18.1 Cleaning
  • 18.2 Decontamination / chemical disinfection of the CO2 incubator
  • 18.3 Hot-air sterilization at 180 °C / 356 °F
    • 18.3.1 Overview
    • 18.3.2 Performing a hot-air sterilization
    • 18.3.3 Aborting the hot-air sterilization prematurely
    • 18.3.4 End of the sterilization cycle
• 19. Maintenance and service
  • 19.1 Maintenance intervals, service
  • 19.2 Checking the air jacket heating fan
  • 19.3 Checking the humidity system fan
  • 19.4 Gas inlet fine filter
  • 19.5 Sending the unit back to BINDER GmbH
• 20. Disposal
  • 20.1 Disposal of the transport packing
    • 20.1.1 Outer unit packing
    • 1.1.1 Packing inside the unit and equipment
  • 20.2 Decommissioning
  • 20.3 Disposal of the unit in the Federal Republic of Germany
  • 20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany
  • 20.5 Disposal of the unit in non-member states of the EC
• 21. Troubleshooting
  • 21.1 Heating
  • 21.2 Gas cylinder pressure too low
  • 21.3 Gas concentration
  • 21.4 Sterilization
  • 21.5 Humidity
  • 21.6 Controller
  • 21.7 Open door
• 22. Technical description
  • 22.1 Factory calibration and adjustment
  • 22.2 Over current protection
  • 22.3 Definition of usable volume
  • 22.4 CB technical data
  • 22.5 Equipment and Options
  • 22.6 Spare parts and accessories
  • 22.7 Important conversion data for non-SI units
  • 22.8 Conversion table for gas inlet pressures, bar – psi
  • 22.9 Dimensions CB 60
  • 22.10 Dimensions CB 160
  • 22.11 Dimensions CB 220
• 23. Contamination clearance certificate
  • 23.1 For units located outside North America and Central America
  • 23.2 For units in North America and Central America
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