Document Outline

• 1 Bio-Plex Suspension Array System Overview
  • Components
  • Advantages
  • For More Information
  • Bio-Rad Technical Support
• 2 Bio-Plex Manager™ Software Overview
  • Software Editions
  • Software Licenses
  • Compatibility with Luminex Software
  • General Workflow
    • Quick Guide
  • Types of Files
  • Key Software Features
• 3 Software Installation
  • System Requirements
  • Required Screen Resolution
  • Installing the Software
    • Microsoft.NET
    • Bioplexdata.mdb File
    • Luminex LXR Directory and Files
  • Hardware Protection Key (HASP Key)
  • Uninstalling
• 4 Starting the System
  • Starting Bio-Plex Manager
    • Connecting with the Array Reader and Microplate Platform
    • Communication between the Array Reader and Microplate Platform
    • Disconnecting and Reconnecting
    • Disconnecting with Sleep Mode
  • Menu and Toolbars
  • Status Bar
  • Quick Guide
  • Sample Needle Adjustment
• 5 Controlling the System
  • Bio-Plex MCV Plate IV
  • Start Up
  • Optics Warm Up and Shut Down
  • Calibration
    • Opening the Calibration Dialog Box
    • Selecting/Entering Calibration Control Numbers
    • Setting Up the Calibration
    • Performing the Calibration
    • Logging the Calibration
  • Start Up and Calibrate
  • Wash Between Plates
  • Remove Air Bubbles
  • Unclog
  • Validation
    • Validation Kit
    • Validation Kit Control Number
    • Setting Up a Validation Run
    • Control Number Selection
    • Validation Type Selection
    • Performing a Validation Run
    • Validation Results
    • Validation Log
  • Platform Heater
  • Instrument Information
  • Eject/Retract Plate
  • Additional Instrument Functions
    • Cancel Operation
  • Shut Down
  • Instrument Operations Log
• 6 Preparing Protocols
  • Protocol Files
    • Creating/Opening Protocol Files
    • Saving Protocols
    • Reducing the File Size of Protocols
  • Multi-Assay Protocols
    • Uses for Multi-Assay Protocols
    • Creating a Multi-Assay Protocol
    • Changing a Protocol from Single to Multi-Assay
  • Protocol Window
    • Step 1. Describe Protocol
    • Step 2. Select Analytes
    • Step 3. Format Plate
    • Plate Groupings
    • Step 4. Enter Standards Info
    • Standard Lots
    • Step 5. Enter Controls Info (Optional)
    • Step 6. Enter Sample Info (Optional)
• 7 Running Protocols
  • Run Protocol Window
  • Bead Count
  • Sample Timeout
  • Reservoir Functions
    • Skip Functions
  • Standard Curve Optimization
  • Advanced Run Settings
    • Bead Map Selection
    • Sample Size
    • Save Options
    • Sampling Errors
    • Doublet Discriminator Gates
  • Plate Loading Guidelines
  • Running the Protocol
    • Status Bar
  • Manually Stopping a Reading
  • Generating Results from a Protocol
  • Histogram and Bead Map
    • Histogram
    • Bead Map
    • Bead Map Display Options
  • Raw Data Table
    • Bead Statistics
    • Sampling Error Codes
    • Raw Data Display Options
    • Set Number Format
    • Other Table Formatting
    • Copying the Raw Data
  • Rerun/Recovery Mode
• 8 Using the Security Edition
  • Bio-Plex Manager™ Security Edition
    • Background on 21 CFR Part 11
    • Standard Mode vs. Secure Mode
    • System Requirements
  • Installing and Starting Bio-Plex Manager Security Edition
  • Users, Passwords, and User Levels
    • User Level Restrictions
    • User Information
  • Enabling and Disabling Secure Mode
  • User Authentication
  • Electronic Records
    • File Security and Validation
  • Calibration, Validation, and Instrument Operations Logs
    • Secure Protocol and Results Files
    • Unsigned Protocol and Results Files
    • Signed Protocol and Results Files
    • Document ID Number and Signature
    • Adjusting the Number of Unknown Samples in a Protocol
    • Generating a Results File from a Protocol File
  • Audit Trail
    • Viewing the Audit Trail
    • Protected Directories
  • Locking Bio-Plex Manager
    • Logging Off
• 9 Analyzing the Results
  • Results Files
    • Opening a Results File
    • Saving a Results File
    • Reducing the Size of a Results File
    • Results Window
    • Custom Reports
    • Viewing/Changing the Protocol Settings
    • Changing the Doublet Discriminator Gate Range
  • Raw Data
    • Setting the Plate ID
    • Exporting the Raw Data
    • Raw Data Table Error Codes
    • Printing the Raw Data
  • Report Table
    • Toolbar
    • Display Options
    • Report Table Column Descriptions
    • Obs/Exp * 100 Column
    • Concentration in Range Column
    • Showing/Hiding Outliers
    • Resizing the Columns
    • Context Menus
    • Sorting Report Table Data
    • Copying the Report Table
    • Printing the Report Table
    • Exporting the Report Table
    • Export Format
    • Export a Subset of the Data
    • Export Preferences
    • Use Multiple Worksheets
    • Exclude Standard Curves
    • Exclude Column Headers/Footers
    • Exclude Table Error Codes
  • Standard Curve
    • Regression Methods
    • Linear vs. Logistic Regression Methods
    • Copying the Standard Curve
    • Exporting the Standard Curve
    • Printing the Standard Curve
  • Graphing Function
    • Choosing Graph Options
    • Viewing Information within Graphs
    • Adding New Graphs
    • Editing an Existing Graph
    • Deleting a Graph
    • Exporting Graphs to Other Applications
    • Adjusting Your Graphs
    • Printing Graphs
  • Document Export Options
    • Bio-Plex XML Export
    • Output CSV File Format
    • Using Stylesheets
    • Command Line Export
    • Exporting the Document
  • Importing Luminex xPONENT Output Files
  • Exporting Results Data to Bio-Plex Data Pro™
• 10 Standard Curve Optimizer
  • Background
    • Standard Curve Recovery
    • Concentration in Range
  • Determining Outliers
    • Recommended Use
    • Standard Curve Optimizer Report
    • Residual Variance and Fit Probability
    • Logistic Weighting
• 11 Data Normalization
  • Normalization Settings
  • Selecting Internal Controls/Housekeeping Genes
  • Assigning a Control Sample
  • Report Table Options
  • Predefined Normalization Report Schemes
  • Normalization Definitions
  • Calculations Based Upon Your Settings
  • Normalization Factor Calculations
• Troubleshooting
• Appendix
  • Security Edition: User Access by Function
  • Normalization Formulas
    • Norm Ratio
    • Norm Ratio’
    • Norm Ratio’’
    • Normalized Fluorescence Values (Norm FI)
    • Ratio (Simple) and the Grouping Function
  • Basic Concepts
    • Microspheres
    • Reporter Fluorochromes
    • Fluidics
    • Excitation
  • Microsphere Handling
    • Microsphere Dispersion
    • Probe Sonicator
    • Bath Sonicator
    • Enumeration of Microsphere Suspensions
    • Microsphere Separation Methods
    • Microsphere Agitation During Assay
    • Microsphere Stability and Storage
  • Software Warranty
  • Reporting Problems to Bio-Rad
  • Report Table Error Codes
• Glossary
• Index