Document Outline

• Chapter 1: Criterion Precast Gels
  • 1.1 Introduction
  • 1.2 Gel Formulations
  • 1.3 Comb Configurations
  • 1.4 Specifications
  • 1.5 Storage Conditions
  • 1.6 Important Notes
• Chapter 2: Setup and Basic Operation
  • 2.1 Workflow Overview
  • 2.2 Required Materials
  • 2.3 Setting Up and Running Criterion Gels in the Criterion Cell
  • 2.4 Removing the Gel
• Chapter 3: SDS-PAGE
  • 3.1 Introduction
  • 3.2 Criterion Gel Selection Guide for SDS-PAGE
    • 3.2.1 Criterion TGX and Criterion TGX Stain-Free Gels
    • 3.2.2 Criterion Tris-HCl and Criterion Stain Free Gels
    • 3.2.3 Criterion XT Bis-Tris Gels
    • 3.2.4 Criterion XT Tris-Acetate Gels
  • 3.3 SDS-PAGE Buffers
    • 3.3.1 Running Buffers
    • 3.3.2 Sample Buffers
  • 3.4 Sample Preparation
  • 3.5 Running Conditions
• Chapter 4: Native PAGE
  • 4.1 Introduction
  • 4.2 Criterion Gel Selection Guide for Native PAGE
    • 4.2.1 Criterion TGX and Criterion TGX Stain-Free Gels
    • 4.2.2 Criterion Tris-HCl and Criterion Stain Free Gels
    • 4.2.3 Criterion XT Tris-Acetate Gels
  • 4.3 Native PAGE Buffers
  • 4.4 Sample Preparation
  • 4.5 Running Conditions
• Chapter 5: Criterion Stain Free System
  • 5.1 Introduction
  • 5.2 Criterion Stain Free Workflow
  • 5.3 Electrophoresis with Criterion TGX Stain-Free Gels
  • 5.4 Using the Gel Doc EZ Imager
• Chapter 6: Peptide Analysis
  • 6.1 Introduction
  • 6.2 Criterion Tris-Tricine/Peptide Gels
    • 6.2.1 Gel Composition
    • 6.2.2 Gel Selection Guide
  • 6.3 Tris-Tricine/Peptide Buffers
  • 6.4 Sample Preparation
  • 6.5 Running Conditions
• Chapter 7: Isoelectric Focusing (IEF)
  • 7.1 Introduction
  • 7.2 Criterion IEF Gels
    • 7.2.1 Gel Composition
    • 7.2.2 Gel Selection Guide
  • 7.3 IEF Buffers
  • 7.4 Sample Preparation
  • 7.5 Running Conditions
• Chapter 8: Protease Analysis by Zymogram PAGE
  • 8.1 Introduction
  • 8.2 Criterion Zymogram Gels
    • 8.2.1 Gel Composition
    • 8.2.2 Gel Selection Guide
  • 8.3 Zymogram Buffers
  • 8.4 Sample Preparation
  • 8.5 Running Conditions
• Chapter 9: Nondenaturing Nucleic Acid PAGE
  • 9.1 Introduction
  • 9.2 Criterion TBE Gels
    • 9.2.1 Gel Composition
    • 9.2.2 Gel Selection Guide
  • 9.3 Nondenaturing Nucleic Acid PAGE Buffers
  • 9.4 Sample Preparation
  • 9.5 Running Conditions
• Chapter 10: Denaturing Nucleic Acid PAGE
  • 10.1 Introduction
  • 10.2 Criterion TBE-Urea Gels
    • 10.2.1 Gel Composition
    • 10.2.2 Gel Selection Guide
  • 10.3 TBE-Urea Buffers
  • 10.4 Sample Preparation
  • 10.5 Running Conditions
• Chapter 11: 2-D Electrophoresis
  • 11.1 Introduction
  • 11.2 Equilibration
  • 11.3 Agarose Overlay
  • 11.4 Second-Dimension Electrophoresis
• Chapter 12: Detection
  • 12.1 SDS-PAGE and Native PAGE Detection
  • 12.2 Peptide Gel Staining
  • 12.3 IEF Gel Staining
  • 12.4 Zymogram Gel Staining
  • 12.5 TBE Gel Staining
  • 12.6 TBE-Urea Gel Staining
• Chapter 13: Blotting
  • 13.1 Introduction
  • 13.2 Transfer
    • 13.2.1 Transfer Buffers
    • 13.2.2 Wet Transfer Using the Criterion Blotter
    • 13.2.3 Transfer Using the Trans-Blot Turbo System
    • 13.2.4 Semi-Dry Transfer Using the Trans-Blot SD Cell
  • 13.3 Total Protein Blot Stains
  • 13.4 Immunodetection
• Chapter 14: Troubleshooting
• Appendix A: Quick Start Guide
• Appendix B: Buffers
• Appendix C: Related Literature
• Appendix D: Ordering Information