Document Outline

• FemtoJet® 4i
• Table of contents
• 1 Operating instructions
  • 1.1 Using this manual
  • 1.2 Danger symbols and danger levels
    • 1.2.1 Danger symbols
    • 1.2.2 Danger levels
  • 1.3 Symbols used
• 2 Product description
  • 2.1 Delivery package
    • 2.1.1 Delivery package – Accessories
  • 2.2 Features
  • 2.3 Exemplary set-up of a microinjection system
  • 2.4 Main illustration
    • 2.4.1 Interfaces
  • 2.5 Name plate
  • 2.6 Control panel
  • 2.7 Rotary knobs
  • 2.8 Foot control
  • 2.9 Hand control
  • 2.10 Pressure parameters
    • 2.10.1 Compensation pressure pc
    • 2.10.2 Injection pressure pi
    • 2.10.3 Injection time ti
    • 2.10.4 Rinsing pressure
    • 2.10.5 Operating pressure pw
  • 2.11 Self-calibration
  • 2.12 Universal capillary holder
• 3 Safety
  • 3.1 Intended use
  • 3.2 Warnings for intended use
  • 3.3 Warning signs on the device
  • 3.4 User profile
  • 3.5 Information on product liability
• 4 Installation
  • 4.1 Preparing installation
    • 4.1.1 Complaints about damages
    • 4.1.2 Incomplete delivery
  • 4.2 4.2 4.2 Selecting the location
  • 4.3 Connecting the Microinjector
  • 4.4 Connecting an external device
    • 4.4.1 Connecting a micromanipulator
    • 4.4.2 Connecting the PC
    • 4.4.3 Connecting two devices
  • 4.5 Connecting accessories
    • 4.5.1 Connecting a foot control
    • 4.5.2 Connecting a hand control
• 5 Software
  • 5.1 Main screen
  • 5.2 Main menu
  • 5.3 Navigating the menu
    • 5.3.1 Selecting the menu and navigating
    • 5.3.2 Exiting the menu
    • 5.3.3 Selecting parameters
    • 5.3.4 Changing a parameter value
• 6 Operation
  • 6.1 Switching on or off the Microinjector
    • 6.1.1 Switching on the Microinjector
    • 6.1.2 Switching off the Microinjector
  • 6.2 Switching on or off the standby mode
    • 6.2.1 Switching on the standby mode
    • 6.2.2 Switching off the standby mode
  • 6.3 Determining the injection parameters
    • 6.3.1 Filling the capillaries with fluorescence dye
    • 6.3.2 Carrying out a test injection
    • 6.3.3 Possible sources of error - cell inflates or bursts
    • 6.3.4 Possible sources of error - capillary is clogged
    • 6.3.5 Possible sources of error - liquid is not being injected
    • 6.3.6 Possible sources of error - capillary does not reach the cell
    • 6.3.7 Result – the injection parameters have been determined
  • 6.4 Setting the compensation pressure pc
  • 6.5 Setting the injection pressure pi
  • 6.6 Setting the injection time ti
  • 6.7 Setting the injection mode
    • 6.7.1 Setting the automatic injection mode
    • 6.7.2 Setting the manual injection mode
  • 6.8 Injecting liquid
    • 6.8.1 Automatically injecting liquid
    • 6.8.2 Manually injecting liquid
  • 6.9 Replacing the capillary
  • 6.10 Rinsing the capillary
  • 6.11 Setting the injection counter to zero
  • 6.12 Calling up saved injection parameters
    • 6.12.1 Calling up saved injection parameters
  • 6.13 Saving or changing the injection parameters
    • 6.13.1 Saving injection parameters
    • 6.13.2 Changing the saved injection parameters
  • 6.14 Adjusting the device settings
    • 6.14.1 Change capillary function – Changing the capillary
    • 6.14.2 Pressure unit function – Selecting the pressure unit
    • 6.14.3 Beeper function – Switching on/off the signal tone
    • 6.14.4 Contrast function – Setting the display contrast
    • 6.14.5 Illumination function – Switching on/off the display illumination
    • 6.14.6 Continuous flow function – Setting the constant operating pressure
    • 6.14.7 Drain pressure supply function – Draining the pressure reservoir
  • 6.15 Inserting the capillary into the capillary holder
  • 6.16 Inserting Femtotips into the capillary holder
• 7 Troubleshooting
  • 7.1 General errors
  • 7.2 Error messages
    • 7.2.1 Error 01 – 10
    • 7.2.2 Error 11 – 18
    • 7.2.3 Error 19 – 38
    • 7.2.4 Error 39 – 40
• 8 Maintenance
  • 8.1 Replacing the sealings in the grip head
    • 8.1.1 Removing the sealing washer and o-rings
    • 8.1.2 Inserting the o-rings and the sealing washer
  • 8.2 Replacing the sealing in the adapter for Femtotips
    • 8.2.1 Replacing the o-ring
  • 8.3 Replacing fuses
  • 8.4 Cleaning
  • 8.5 Hints with regard to service intervals
  • 8.6 Service and maintenance
  • 8.7 Disinfection/decontamination
• 9 Technical data
  • 9.1 Mode of operation
  • 9.2 Weight/dimensions
  • 9.3 Power supply
  • 9.4 Interfaces
    • 9.4.1 USB
    • 9.4.2 RS 232
    • 9.4.3 Injection time ti
    • 9.4.4 Injection pressure pi
    • 9.4.5 Compensation pressure pc/pw
    • 9.4.6 Rinsing pressure
    • 9.4.7 Accuracy
  • 9.5 Ambient conditions
• 10 Transport, storage and disposal
  • 10.1 Storage
  • 10.2 Decontamination before shipment
  • 10.3 Transport
  • 10.4 Disposal
• 11 Ordering information
  • 11.1 FemtoJet 4i
  • 11.2 Accessories
  • 11.3 Universal capillary holder and accessories
• Declaration of Conformity