GE 71-5000-15 AD User Manual
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1. Product description
CNBr-activated Sepharose 4 Fast Flow is a bead-formed, highly cross-
linked pre-activated matrix produced by reacting Sepharose 4 Fast Flow
with cyanogen bromide (CNBr). This coupling makes the medium more
rigid which in turn improves the pressure/flow characteristics. Proteins
and other molecules containing primary amino groups can be coupled
directly to the pre-activated medium. Multi-point attachment of proteins
provides the immobilized product with good chemical stability. The resulting
affinity medium can isolate a specific substance from a complex mixture,
often achieving very high yield and purity in a single step. Many references
demonstrate that binding affinity is frequently well maintained after CNBr
coupling.
A typical application of pre-activated affinity media like CNBr-activated
Sepharose 4 Fast Flow is based on antigen-antibody reactions with
immobilized monoclonal antibodies as ligands. In such cases, purification
factors of 2 000–20 000 can be obtained.
Table 1 summarizes the main characteristics of CNBr-activated Sepharose 4
Fast Flow.
Table of contents
1. Product description
2
2. Coupling
3
3. Column packing guidelines
5
4. Evaluation of packing
7
5. Cleaning, Sanitization and Storage
10
6. Ordering information
11