Ocean Optics Red Tide Guide - Manual (Page 8)

170-00000-RT-02-1108 8
Next, store a dark spectrum. Either switch off the lamp, or
place an opaque object in the cuvette holder to block the
light path. Then click the Store Dark button.
Note:
The lamp must be ON before you click the Finish button.
Fig. 20
Click Finish.
A dialog box appears giving you three choices. See
Fig. 21.
•
Beer-Lambert Law
• Calibrate from solutions of known concentration
• Load calibration from file
Notice that an Absorbance graph is running in the
chart window. See Fig.21.
Fig.21
BEER-LAMBERT LAW
Click the radio button for Beer-Lambert Law.
Enter the data. The value of ε will either come from a
reference text or from the experiments with known con-
centrations described in the next section.
Click Finish to see the concentration reading. Now you
can use any concentration in a cuvette and get an in-
stant concentration reading by clicking Single Update or
Continuous Update.
To run another concentration measurement use
File>New>New Concentration Measurement
, but use
Active Processing because you have an active
absorbance graph already running.
Fig. 22
Fig. 23