Carolina 3M Petrifilm E.Coli/Coliform Count Plates - Manual (Page 2)

Description
The 3M™ Petrifilm™ E. coli/Coliform Count (EC) Plate is a ready-made
culture medium system designed to facilitate counting coliforms and E. coli.
Petrifilm EC Plates contain nutrients, a cold-water-soluble gelling agent, and
indicator dyes. Petrifilm plates are prepared on a grid background to
facilitate counting colonies.
Petrifilm EC plates are designed to allow organisms to grow in an oxygen-
limited environment. Their system of nutrients and dyes enables the user to
identify colonies formed by coliforms and by E. coli. For more information,
please see Interpretation. Petrifilm plates can be used in a variety of ways:
• Petrifilm plates can be hydrated with a bacterial culture, or a dilution of a
culture, for counting the viable organisms present. See Method A.
• Petrifilm plates can be hydrated first with water or buffer, then inoculated
by swabbing, streaking, or touching to surfaces. See Method B.
• Antibiotics can be added to the hydration fluid to select for resistant
organisms in Method A or B.
• Cells can be removed from colonies growing on Petrifilm plates and used
to inoculate additional cultures or for staining.
• Experimental results on Petrifilm plates can be saved for future reference
by scanning the plates on a standard computer-linked scanner. Do not
open the plates for scanning.
Petrifilm plates were developed for use in the food and beverage industry.
They have been certified for official analyses in many countries. For more
information about these applications, see the 3M page on the World Wide
Web at www.3m.com.
©2000 Carolina Biological Supply Company Printed in USA
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