Carolina C-FERN Spores - Manual (Page 7)

c. To evenly suspend the spores, bubble air through a clean, sterile
pipet.
d. Sterilize the spores for 3 minutes.
e. Remove the sodium hypochlorite solution with a clean, sterile pipet.
• Rinse the spores:
a. Add 1 full pipet (2 mL) of sterile distilled water.
b. Remove the water with a clean, sterile pipet.
c. Repeat 1 or 2 more times.
Sowing and Spreading Spores
Sowing Spores
For consistent coverage of the petri dishes, the spores must be suspended
before each sowing.
To suspend the spores,
• gently draw the liquid, along with the spores, in and out of a pipet.
• withdraw a small amount of the spore suspension into the pipet and
immediately dispense the appropriate number of drops onto the agar
(300+ spores = 3 drops of spore suspension per dish).
When sowing,
• tilt the lid of the petri dish upward only enough to permit access of the
pipet.
• hold the pipet at a constant angle of 45°.
• do not touch the agar surface with the tip of the pipet.
• resuspend the spores between each sowing by gently squeezing and
releasing the pipet bulb.
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