Luminex MasterPlex QT Tutorial Manual - Manual (Page 23)

http://www.miraibio.com 23 Master Plex QT
For analyte IL-5, the starting concentration is 15,900 pg/mL, and the dilution factor is 3
(since it is a 1:3 dilution). Note the various arrow keys – these are used to indicate the
direction of the dilution; that is, the direction you would move as if you were doing the
serial dilution in a 96-well plate, moving from most concentrated to least concentrated.
The blue arrows and the red arrows allow you to specify the direction if the dilution spans
more than one column or one row, respectively. The green arrows are used if your
standards lie entirely in either one row or one column. The most concentrated well is B1,
and the serial dilutions proceed down to H1, so we’ll select the green down-arrow key.
For IL-5, the window should appear as shown:
Click Fill, and the following dialog box will appear:
Click OK, and then click Cancel/Exit in the Auto Fill dialog box.