Penn-Century IA-1B-R for Rat - Manual (Page 7)

7
Removing all air bubbles from the syringe
The Penn-Century MicroSprayer® Aerosolizer is an air-free aerosol system. Air bubbles will cause the
quality of the spray to degrade. After loading the syringe, turn it with the distal tip pointed upward and
push the plunger to force any air bubbles out the distal tip. Always push out any air bubbles trapped in
the syringe prior to use.
Determining the optimal dose range for your applications
· Direct aerosol administration to the lungs with a Penn-Century intratrachael device permits far
more precise quantification of the delivered dose and far higher drug concentration than is
possible with large nebulizer or exposure systems for animals that mix drug with compressed air
that is blown - at high momentum - at the nose of the animal. The aerosol from the
MicroSprayer® Aerosolizer is gentle, air-free, low momentum, highly concentrated and precisely
quantifiable.
· A review of literature citing Penn-Century devices indicates that they permit far more efficient
administration of very small doses, as well as the ability to deliver far larger doses volumes than
would be possible by animal nebulization/exposure/inhalation systems and more far more safely
than liquid bolus/droplet instillation through a gavage needle, catheter or endotracheal tube.
· Researchers should take into consideration that the optimal, most effective dose
range they can administer using a Penn-Century intratracheal drug delivery device
may be an amount that is far smaller or far larger than is possible within the
limitations of standard methods of pulmonary drug delivery in animal models. The
user must conduct feasibility experiments to determine the optimal dose for their own
formulation by increasing the dose in small increments to determine the minimum or maximum
effective dose, and to plan for this process in their experimental design.