Techne TC-3000G - Manual (Page 50)

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NOTES ON TRANSFERRING PROTOCOLS FROM OTHER THERMAL CYCLERS
The TC-3000G can heat up to 3.3°C/s and provides the highest levels of temperature uniformity. Many
slower machines have historically begun the countdown on hold times before the set temperature is
reached in order to avoid any overshooting in sample temperature. The slower the heating rate the more
significant the loss in hold time, i.e. if a 30 second hold time is set at 55°C then perhaps 5 to 10s will be
lost in between the start of the time countdown and actually reaching the set temperature. The overall
effect of this method of control is that your sample, if cooling from a higher temperature, is subjected to
an environment with an elevated temperature compared to the target temperature for a significant part
of the hold time.
The TC-3000G provides the user with 'what-you-program-is what-you-get'. Due to the rapid and
responsive nature of the Peltier heating system, the time between the timer countdown initiating and
actually reaching the set temperature has been reduced to as little as 1s. This ensures that the sample is
only subjected to the exact temperature required.
Taking the above into consideration it must therefore be noted that care must be taken when re-
optimizing protocols from a slower machine.
Trouble Shooting
1. The optimum annealing temperature may in fact be higher than the set temperature used in the slower
machine. Try increasing it by 1 or 2°C.
2. The hold time can be reduced once the optimum temperature has been found. Try reducing it by 5 to
10s.