Eppendorf Concentrator plus - Manual (Page 25)

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5 Operation
Concentrator plus / Vacufuge® plus — Operating manual
5.4 Concentration
Requirement
The prerequisite for all applications described here is the previously described preparation (see
Preparation for concentration on page 24).
5.4.1 Concentration with time setting
Perform the following steps in the sequence described.
1. time arrow keys: set running time.
2. brake: switch brake on or off.
3. temp: select temperature (note temperature resistance of the samples).
4. mode/vent: select mode V-AQ, V-AL or V-HV .
5. start/stop: start concentration.
• The device lid is locked, the green indicator lamp lid goes out.
• The rotor starts up.
• At 1,000 rpm the vacuum pump switches on, the indicator lamp vac lights up.
• The vent valve is closed.
• The rotor accelerates to the end point of 1,400 rpm.
• On the display, the colon of the time display flashes while the rotor is turning.
• The remaining run time is displayed in hours and minutes.
When concentration is completed
• The device stops automatically.
• During braking the elapsed centrifugation time is displayed flashing.
• The rotor chamber is ventilated so that the pressure in the chamber increases slowly.
• After two seconds, the vacuum pump is switched off, the indicator lamp vac goes out.
• Then the device brakes.
• When the rotor stands still, the indicator lamp lid lights up.
• The lid can be opened.
6. Remove samples.
WARNING!
Warning! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!
 Before starting concentration, follow the safety instructions relating to hazards from
unsymmetrically loaded and/or overloaded rotors and from overloaded and/or damaged tubes
(see Warnings for intended use on page 15).
WARNING!
Warning! Risk of injury from improperly attached rotors.
 Only operate the device if the rotor has been mounted properly.
 If there are any unusual noises when the device is started up, the rotor may not be properly
attached. Stop the concentration immediately by pressing the start/stop start/stop key.
CAUTION!
Caution! Overflowing of the emission condenser.
The max. volume of the emission condenser is 280 ml.
1. Check before each run whether the volume of the emission condenser is sufficient for your
application.
2. Empty the emission condenser, if required (see Emptying the emission condenser on
page 29).
EN
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ing manual