Eppendorf BioPhotometer plus - Manual (Page 34)

7 Maintenance
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BioPhotometer plus — Operating manual
7.5 Check photometer
To check the photometric accuracy and the accuracy of the wavelength Eppendorf offers a filter
kit (secondary UV-VIS filter). The kit contains three filters ("sample A1", "sample A2" and "sample
A3") to check the photometric acccuracy and two filters ("sample 260 nm" and "sample 280 nm")
to check the accuracy of the wavelength. The absorbances of the filters are measured against a
blank filter ("blank A0"). In addition to information about the accuracy you are also provided with
information about the precision: from the 10 measurements each per wavelength the CV value is
calculated in addition to the mean value.
To perform a blank measurement, firstly blank filters are inserted like cuvettes into the cuvette
shaft followed by the test filters. The absorbance values measured for the test filters are
compared to the permitted value range. The limit values for the permitted range for the individual
filters are printed in a table in the lid of the filter box (see in table: "X.XXX – X.XXX E").
Tab. 3: Inside lid of the filter box (sample)
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