C.B.S. Scientific TTGE-2401 - Manual (Page 10)

ClearPAGE Precast Gel Running Instructions 7-1-14 10
BUFFER FORMULATIONS
As an alternative to the buffers ClearPAGE sells, these formulations may be used to prepare buffers
yourself. Use high-quality, low-conductance ingredients. Do NOT use acid or base to adjust the pH!
Standard SDS Running Buffer, 20X for Reduced Samples (FB60500)
Ingredient MW Molarity Qty/Liter
Tricine (free acid) 179.17 0.8M 143.4 g
Tris (free base) 121.14 1.2M 145.2 g
SDS (2%) 288.38 - 20.0 g
Sodium Meta-bisulfite 104.06 50mM 5.0 g
Ultrapure water (fill to) - - 1000ml
* pH should be between 8.4 and 8.5 at 25° C.
* For non-reduced samples (especially antibodies), omit the Sodium Meta-bisulfite
Turbo SDS Running Buffer, 20X FB13500
Ingredient MW Molarity Qty/Liter
MPS (free acid) 209.26 0.6M 125.6 g
Tris (free base) 121.14 1.2M 145.2 g
SDS (2%) 288.38 - 20.0 g
Sodium Meta-bisulfite 104.06 50mM 5.0 g
Ultrapure water (fill to) - - 1000 ml
* pH should be between 8.3 and 8.4 at 25° C.
* For non-reduced samples (especially antibodies), omit the Sodium Meta-bisulfite
LDS Sample Buffer, 4x -FB31010
Ingredient MW Molarity Qty/Liter
Glycerol (40%) - - 400 g
Ficoll-400 (4%) - - 40 g
Triethanol amine, pH7.6 149.2 0.8M 120.0
6 N HCL 36.46 - 93.0 g
Lithium Dodecyl Sulfate (4%) - - 40 g
EDTA Di-Sodium 372.2 2mM 7.44 g
Brilliant Blue G250 (0.025%) - - 0.25g
Phenol Red - - 0.25 g
Ultrapure water (fill to) - - 1000 ml
* pH should be between 7.7 and 7.8 at 25° C.