C.B.S. Scientific DCX-900C - Manual (Page 13)

13 www.cbsscientific.com
5. Place the notched plate on top of the bottom
assembly, starting from the bottom edge and
gently easing the plate down. Verify the gasket
is smooth around the edges and then clamp
along the bottom.
6. Lift the assembly and stand it on the base
of the clamps. For leveling, push glass plate
assembly down until it stops against clamp
body. Clamp the sides of the assembly with
additional casting clamps on either side. As
each clamp is attached, be sure the gasket is
aligned between the plates forming a seal.
7. Apply PAGE or agarose solution to gel plate
sandwich using a syringe or pipette. If using
a stacking gel, pour desired height of running
gel, then overlay a small amount of dH2O
or 0.1% SDS solution to top of gel. After
polymerization, rinse with buffer, add stacking
gel solution and insert comb. For regular, unit
percentage gels, add polyacrylamide solution
to correct height, and insert comb. Allow gel
to set, usually 20 minutes. Extra gel solution
in pipette or syringe can be monitored to test
polymerization of gel mix.
8. Disassembly. Hold the clamped plate as-
sembly with one hand. Remove the gasket by
starting at one of the top ends and pulling up
and out on the gasket until it releases from the
plate, up to the bottom of each of the white
clamps. When each clamp is reached DO
NOT remove it, instead feed the gasket down
through the clamp body and repeat pulling up
and out. Continue feeding until the gasket is
fully detached. If gel, is not to be used imme-
diately, wrap entire plate sandwich with plastic
wrap tightly to seal and store at 4°C for up to
a month.