Labnet Biopette A Autoclavable Multichannel Pipettes - Manual (Page 7)

7 8
M U L T I C H A N N E L
• Perform three aspiration series (each series
should include the aspirations from all chan-
nels), weigh each time and calculate the aver-
age value of the aspirations.
• Calculate average aspirated volume in µl mul-
tiplying the average aspiration amount [mg]
by the distilled water density coefficient
[µl/mg], which depends on temperature and
pressure according to the following table:
If the average aspirated volume exceeds the
permissible value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A)
Warning: The pipetting pushbutton consists
of 2 parts: the knob (Fig. 1A2) and the
pushbutton (Fig. 1A1). After removal of the
pushbutton, both parts are separated.
• Holding the volume setting knob to protect
it against rotation, insert the calibration key
into the cuts of the calibration screw, (Fig. 4B)
• Turn the key clockwise to reduce the aspirat-
ed volume, or counter-clockwise to increase
the volume, (Fig. 4C). One full turn of the calibra-
tion key changes the pipette aspiration volume
by the amount given in the table
• Take out the key and fix the pipetting pushbut-
ton (Fig. 4D). The pipetting pushbutton should
be fixed by placing the knob on the arbor first
(Fig. 1A2) and then the pushbutton (Fig. 1A1).
Determine the average aspirated volume. The
average volume should be within the permissi-
ble range given in the table. If the volume
exceeds the values stated, the recalibration pro-
cedure should be repeated.
6 - CLEANING AND STERILIZATION
Cleaning
External surfaces of the pipetting pushbutton,
the ejector button, the handgrip, shaft and the
adjustment knob may be cleaned using a cloth
dampened in isopropyl alcohol.
Sterilization:
The pipette can be sterilized in the autoclave at
121 °C for 20 minutes. After sterilization, the pi-
pette should be dried and cooled to room tem-
perature.
It is recommended:
- to sterilize the pipette in autoclave with an initial
vacuum and drying cycle,
- prior to sterilization unscrew the nut connecting
the handle and module slightly. After autoclav-
ing these parts should be screwed tight again.
The precision of the results should not alter if the
pipetting process and autoclaving are carried out
as described in this manual. Because a slight
change in the accuracy of the dosage may
occur, it is recommended to:
- check the calibration of the pipette after the ini-
tial first, third and fifth autoclaving cycles and
then after every 10 autoclaving cycles.
7 - PIPETTE KIT AND ACCESSORIES
Pipette kit
The pipettes are delivered in the kits including:
• Pipette
• Instruction manual
• Calibration key
• Identification labels
Temperature [°C]
Pressure [kPa]
95 101.3 105
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
BPM/08/2004 v06 31/07/2004 01:06 PM Page 12