Leica Biosystems CM1850 UV - Manual (Page 37)

37 Leica CM1850 UV – Cryostat
Problem Cause Remedy
8. Troubleshooting
Fat on the corner or edge of the
anti-roll plate.
- Rusty knife/blade.
- Static electricity or air cur-
rents.
- Anti-roll plate too warm.
- Temperature too low for the
tissue cut.
- Blunt part, dirt, dust, frost or
rust on the knife/blade.
- Leading edge of anti-roll plate
damaged.
- Hard particles in the tissue.
- Knife back dirty.
- Microtome was not entirely dry
when switching on refrigera-
tion; consequently ice built up
in the micrometer feed system.
- Defective microtome.
- Moisture on the underside
caused the disc to freeze to the
freezing shelf or specimen
head.
- Mains plug not properly con-
nected.
- Defective fuses.
- Temperature control switch
activated.
Flattened sections curl up when
anti-roll plate is picked up
Sections tear
Inconsistent or insufficient speci-
men feed
Specimen disc cannot be re-
moved
Cryostat inoperational
- Remove fat with alcohol.
- Remove rust.
- Remove static electricity.
- Cool down the anti-roll plate.
- Increase temperature and
wait.
- Remove cause.
- Replace the plate.
- - -
- Clean.
- Remove the microtome and
dry it thoroughly before rein-
stallation.
- Call technical service.
- Apply concentrated alcohol to
the contact point or heat the
specimen head.
- Check the mains plug is prop-
erly connected.
- Replace the fuses.
- Check site conditions as de-
scribed in section 5.2, and
reset the temperature control
switch.