UVP Crosslinkers - Manual (Page 7)

UV CROSSLINKERS UVP
7
APPLICATIONS
The Crosslinker is a multi-purpose ultraviolet exposure instrument for use in the laboratory. A wide
variety of applications for ultraviolet radiation exist in the laboratory.
A. UV crosslinking of DNA and RNA by covalently binding nucleic acids to transfer membranes
nitrocellulose, nylon or nylon-reinforced nitrocellulose membranes after Northern, Southern, slot
or dot blotting.
The PRESET ULTRAVIOLET EXPOSURE SETTING is factory set to a UV dose of 120,000
microjoules per cm² for this laboratory purpose. This setting has been found to be the optimal
dose for DNA retention and hybridization-signal sensitivity.
B. Nicking ethidium-bromide stained DNA in Agarose Gels
C. Gene Mapping for creating cleavage-inhibiting thymine dimers
D. Testing RecA function
E. Ultraviolet Sterilization
F. Elimination of PCR contamination
G. UV Curing
H. Fluorescence of materials
Further Applications can be achieved with the Crosslinkers by changing the ultraviolet
wavelength (see Section Changing the UV Wavelength).
MAINTENANCE, CARE AND CLEANING OF THE CROSSLINKER
The UVP UV Crosslinker is built to provide you with trouble-free operation. To ensure correct service:
Note: ALWAYS UNPLUG UNIT FROM ELECTRICAL SUPPLY BEFORE CLEANING OR DRYING.
1.
Wipe ANY water from inside and outside the unit with a soft cloth or sponge.
2.
Use soap and water with a soft cloth or sponge to clean the unit.
3.
Do not allow chemicals to remain on unit surfaces.
4.
Never clean unit with abrasive pads or cleaners.
5.
Never clean unit with acetone or chloroform.
6.
Clean the UV sensor regularly with a soft cloth and alcohol.
UVP offers technical support for all of its products. If you have any questions about product use,
operation or repair, please contact UVP customer service at the following numbers:
Corporate Headquarters: UVP, LLC, 2066 W. 11th Street, Upland, CA 91786
TOLL FREE USA (800) 452-6788, PHONE: (909) 946-3197, FAX: (909) 946-3597
European Operations: Ultra-Violet Products Ltd., Unit 1, Trinity Hall Estate, Nuffield Road
Cambridge CB4 1TG United Kingdom, PHONE: +44(0)1223 420022, FAX:+44(0)1223 420561
Note: A Returned Goods Authorization (RGA) number must be obtained from UVP before returning
any item to UVP.