UVP HM-4000 Multidizer - Manual (Page 3)

Hybridization Ovens
Page 3
Introduction
The HB-1000 Hybridizer is a cost-effective, high-performance, 10-bottle hybridization oven with state-of-the-art
microprocessor temperature and variable speed controls. The HL-2000 HybriLinker™ System is designed with a
combination of hybridizer and crosslinker components. This equipment is intended to be used in the laboratory
environment.
The HM-4000 Multidizer™ System is designed as a multifunction unit with a variety of motion options. The lower
chamber operates in the same manner as the HB-1000. However, the HM-4000 has an additional upper chamber for
the use of a reciprocating shaker tray, an orbital rocker tray, or an acrylic carousel. Refer to the “Accessories” section
of this manual for ordering information.
The HB-1000 Hybridizer can be set to operate anywhere from 10 to 15 RPM, enabling consistent saturation of
samples for washing or hybridizing. Having the ability to remove the rotary carousel wheel and replace it with an
interchangeable rocker plate enables all hybridization protocols in one reliable benchtop unit. An easily removable
protective tray under the carousel allows easy clean-up of spilled media.
A touch-sensitive keypad and microprocessor provide accurate temperature control from ambient 10°C to 99.9°C.
The keypad is located just below a large LED readout. Once the desired hybridization temperature is entered, the
readout displays the current temperature inside the chamber. The chamber environment is calibrated at 68.0°C. At
this temperature, the accuracy is +/-0.5°C. An internal circulating fan assists in uniformly distributing heating
throughout the chamber.
Two different hybridizer bottle sizes are available for use within the hybridizer using the standard carousel provided
with the unit. The rotary carousel wheel can hold up to twenty (20) 35 x 150 mm bottles or ten (10) 35 x 300 mm
bottles. In addition, combinations of both size bottles may be used at the same time. An optional carousel kit for
oversized bottles is available.
There may be build up of pressure within the hybridization bottles when they are taken from ambient to hybridization
temperature. To help in relieving some of this pressure, preheat the solutions and bottles. Also, be sure bottles are
opened at the same temperature at which the hybridization was done. Do not allow the bottles to cool before opening.
To ensure the hybridization bottles remain leak proof and pressure proof, prevent temperatures above 70°C. If
temperatures above this are used without first relieving the pressure within the bottle, there is a risk that bottles will
leak and/or break due to internal pressure build-up.
If the bottles are accidentally taken above 70°C without relieving the pressure, DO NOT open the hybridizer door.
Turn off the hybridizer and allow it to cool before opening the door and checking the contents of the oven. Should the
bottles be used above 70°C, relieve the pressure by unscrewing and re-tightening the cap.
If using Radioactive material, see the “Decontamination” section of this manual.
The HL-2000 HybriLinker System and HM-4000 Multidizer System are designed to be carried or lifted by two
people.