UVP iBox Explorer Imaging Microscope - Manual (Page 15)

iBox Explorer
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15
Preparing to Capture Images
Platform Stage/Warming Plate
The platform stage/warming plate creates a constant temperature of 38ºC to warm and prevent hypothermia in
animal specimens. Slide out the platform stage and place the specimen on the warming plate of the iBox
Explorer
2
. The dark background creates an ideal imaging surface while maintaining a constant temperature for
the animal.
For samples that do not require heat, it is possible to disable the warming component of the platform stage by
moving the switch on the warming plate control box to the OFF position.
Selecting Excitation Filters and Lighting
VisionWorksLS provides automated control of the BioLite Xe excitation filters and lighting via the Lighting
menu. Note that it is possible to control the BioLite Xe manually via the controls on the front of the unit;
however, this is not recommended as any changes made may not be reflected in VisionWorksLS.

Select from one of eight excitation filters (GFP and RFP are included) using the “Filter” drop-down
menu shown.

Select the “On” radio button next to “Light Engine”.

Light intensity is selected via the “Intensity” slider, with intensities ranging from 1 (minimum) to 6
(maximum). The relative outputs for each setting are:
Setting Relative Intensity
6 100%
5 50%
4 40%
3 25%
2 12%
1 0%

Select the illumination path through the “Illumination” menu (either side or coaxial lighting).
Excitation
Filter
Light Intensity
Illumination
Path