UVP High Performance UV Transilluminators - Manual (Page 5)

High Performance UV Transilluminators Page 5
holes on the front of the transilluminator. The cover is adjustable to varying angles
for access to the filter surface.
If you are not using the transilluminator with an imaging system darkroom, do not
operate the unit without securing the cover. If the cover is missing, a UV Blocking
Faceshield must be worn to avoid UV exposure to the skin. UV Blocking Eyewear
should be worn even with the cover in place to avoid accidental UV exposure.
Using the Transilluminator
Place gel/sample on the filter area. It is recommended to place the gels on a Gel-Tray to
protect the filter surface from cuts and scratches. It is recommended that gloves be worn
to avoid contact with gel and staining agents.
Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath
the filter. If using the transilluminator with an imaging system, the system’s main power is
required to be in the ON position.
When using a transilluminator with multiple UV wavelengths, dial the knob to the
appropriate wavelength setting.
When using the Variable Intensity models, use the variable intensity settings as follows:

High: allows for UV excitation of fluorophores on gels for routine photography
and for excitation of gels with low sample concentration

Medium: Excellent for viewing and quick single-band excision

Low: Allows for positioning and preparation of gels, excising multiple bands and
focusing for photography
After viewing/photographing the sample, turn the transilluminator off.
Cleaning and Care of the Transilluminator
Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can
damage the UV filter surface).
To protect the filter glass and minimize moisture and liquids on the glass, it is
recommended that you use a UV transmitting Gel-Tray. Refer to the Replacement Parts
for ordering information.
Replacing Tubes in the Transilluminator
1. Disconnect the transilluminator for the electrical supply.
2. Remove the filter cover: Use a Phillips head screwdriver to remove the four screws
on the sides of the unit. Lift the filter cover off the unit.
3. Remove the reflectors on the left and right side of the unit: Slide the reflectors up out
of the unit.
4. Remove the tube: Carefully rotate the tube and slide out of the socket. Replace with
a new tube by sliding the tube into the socket and rotating into place.
5. Insert the reflectors back into place and reattach the filter cover.