Waters ACQUITY UPC2 Torus Columns - Manual (Page 2)

2
[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Torus Columns Care & Use
The ACQUITY UPC 2 packing materials are designed for use
with the ACQUITY UPC 2 Systems and are manufactured in an
ISO-certified plant using ultra-pure reagents. Each batch of
ACQUITY UPC
2 material is tested and the results are held to
narrow specification ranges to assure excellent, reproducible
performance. Every column is individually tested and a
Performance Chromatogram and Certificate of Batch Analysis
is provided on the eCord™ Intelligent Chip.
ACQUITY UPC 2 Columns are designed and tested specifically
for use on ACQUITY UPC 2 Systems. ACQUITY UPC 2 Columns
will exhibit maximum chromatographic performance and benefit
ONLY when used on holistically-designed ACQUITY UPC
2 Systems
since these systems and columns were created and designed to
operate together. For these reasons, Waters cannot support the
use of ACQUITY UPC
2 Columns on any system other than an
ACQUITY UPC 2 System.
II. GET TING START ED
Each ACQUITY UPC 2 Column comes with a Certificate of Analysis
and a Performance Test Chromatogram. The Certificate of Analysis
is specific to each batch of packing material and includes the gel
batch number, physical characterization, analysis of unbonded
particle, analysis of bonded particles, and a SFC chromatographic
batch test. The Performance Test Chromatogram is specific to
each individual column and contains the following information:
gel batch number, column serial number, USP plate count, USP
tailing factor, capacity factor, and chromatographic conditions
under normal-phase LC conditions. These data should be stored for
future reference.
a. Safety Considerations
SFC columns, while in use, are under pressure with CO
2
and
possible modifiers as a supercritical fluid. A major safety concern
is frostbite caused by adiabatic cooling when CO
2
decompresses
from a fluid to a gas at atmospheric pressure. Pay attention to any
frosting on the column or system connections. This indicates a
leak, usually with temperatures far below 0 °C.
Any small leak could produce a situation where the LEL (lower
exposure limit) is reached. Laboratories should be equipped with
CO
2
and/or O
2
sensors when carbon dioxide (CO
2
) is in use.
b. Column Connectors and Installation
ACQUITY UPC 2 Systems utilize tubing and connectors which
have been designed to meet stringent tolerance levels and to
minimize extra-column volumes. For information on system
tubing and connectors, please refer to the ACQUITY UPC
2 System
Operator’s Guide (Part Number 720004226EN can be found on
www.waters.com/chemcu ).
Note: Scale the flow rate up or down accordingly based upon
the column i.d., length, particle size, and backpressure of the
ACQUITY UPC
2 Column being installed.
1. Make sure your co-solvent pump is primed and has an adequate
solvent/modifier supply before performing injections. It is
recommended to use CO
2
with purity level of 99.97% (food
grade) and use high-quality chromatography grade solvents
(typically methanol) as organic modifier.
2. Connect both the inlet and outlet of the column to the
SFC system.
3. If the column is still filled with a solvent, use a low flow rate
and back pressure setting (100 bar) to start pumping CO
2
and modifier through the column.
4. If you see frosting on the column at the inlet or outlet, tighten
the finger-tight fitting or compression screw on that side. If you
continue to see frosting, turn off the CO
2
and vent the system.
Allow the column to depressurize fully before disconnecting
the inlet or outlet to troubleshoot the leaking issue.
Achiral
Chemistry
Stationary Phase
Particle
Shape
Particle
Size (µm)
Pore Size (Å)
Pore Volume
(cc/g)
Surface Area
(m 2 /g)
Endcapped
Torus 2-PIC 2-Picolylamine Spherical 1.7 130 0.7 185 Proprietary
Torus DEA Diethylamine Spherical 1.7 130 0.7 185 Proprietary
Torus Diol High-density Diol Spherical 1.7 130 0.7 185 Proprietary
Torus 1-AA 1-Aminoanthracene Spherical 1.7 130 0.7 185 Proprietary
Table 1. Physical Characteristics.