Waters Quantitative Peptide Retention Standard - Manual

[ CARE AND USE MANUAL ]
Quantitative Peptide Retention Standard
TABLE OF CONT ENTS:
I. INTRODUCTION
II. STORAGE AND STABILITY
III. RECONSTITUTION OF THE PEPTIDE
RETENTION STANDARD
IV. EXAMPLE OF USING THE PEPTIDE
RETENTION STANDARD ON THE
ACQUITY UPLC
V. ORDERING INFORMATION
I. INTRODUCTION
This standard was specifically designed and formulated as a simple peptide
mixture that provides a broad retention window for a variety for a variety of
peptide separation applications. It is an ideal reference material since the
peptides were designed to be stable and to not be prone to modification such
as oxidation.
This standard complements it’s qualitative counterpart, the MassPREP™
Peptide Standard which offers a versatility of various peptides but it not
quantitative. Each peptide in the Quantitative Peptide Retention Standard
is quantitated via amino acid analysis to provide greater accuracy to each
analysis, has been verified by mass identification and is highly pure (more
than 95% pure). Each standard is provided in a Waters TruView™ Maximum
Recovery Vial which simplifies the sample preparation process and allows
for direct injection once solubilized.
The standard consists of four neutral peptides with a single A280
chromophore and varying hydrophobicities and is formulated such that
the peptide components are present in equal abundance. See Table 1 for
specific peptide information.
Table 1: Peptide Mixture Components and Example Data Generated
from Figure 1
Peptide Retention Standard
Peak Area
Peptide MWmono RT (min)* (A
280
) % Area
DG Y GK 538.2387 2.6 2446 24
DTVG Y GVK 837.4232 10.5 2572 25
DFVG Y GVK 883.4440 13 2801 27
DFVG Y GVKDFVGVGVK 1684.8825 16.4 2437 24
280 nm chromophore ( Y ) highlighted
* See Figure 1 for retention time examples.
Applications of the standard can include, but are not limited to: evaluation
of columns and LC systems dedicated to peptide analysis as well as
monitoring performance and recovery across romatographic gradients.