Waters Protein-Pak PW Ion-Exchange Column - Manual

[ Care and Use ManUal ]
Protein-Pak PW Ion-Exchange Columns 1
I. INTRODUCTION
Waters Protein-Pak ™ PW ion-exchange packings are a fully porous 10 μm
hydrophilic rigid gel providing excellent resolution and recovery of aqueous
biomolecules such as proteins, peptides, enzymes and nucleic acids. Three
stainless steel and two glass columns are available as shown in the fol-
lowing description.
Protein-Pak DEAE-5PW 7.5 mm x 75 mm, Steel (P/N WAT088044)
Protein-Pak DEAE-5PW 8.0 mm x 75 mm, Glass (P/N WAT011783)
This ion-exchange packing is prepared by introducing diethylaminoethyl
groups onto a hydrophilic rigid resin. The large pore size of 1000 Å allows
separation of biomolecules up to a molecular weight of 10
6 . Columns are
shipped in distilled water.
Protein-Pak SP-5PW 7.5 mm x 75 mm, Steel (P/N WAT088043)
Protein-Pak SP-5PW 8.0 mm x 75 mm, Glass (P/N WAT011784)
The cation-exchange column has a sulfopropyl group introduced onto a
10 micron hydrophilic rigid resin. The large pore size of 1000 Å allows
separation of biomolecules up to a molecular weight of 10
6 . Columns are
shipped in distilled water.
Protein-Pak SP-5PW 21.5 mm x 150 mm, Steel (P/N WAT010640)
The cation-exchange column has a sulfopropyl group introduced onto
a13 μm hydrophilic rigid resin. The large pore size of 1000 Å allows
separation of biomolecules up to a molecular weight of 10
6 . Columns are
shipped in distilled water.
Note: The loading capacity will be approximately 10 - 20 times greater with
the 21.5 mm x 150 mm compared to the 7.5 mm x 75 mm column.
CONTeNTs
I. INTRODUCTION
II. PRePaRaTION fOR OPeRaTION
a. Steel Column Installation
b. Glass Column Installation
c. Solvent Requirements
d. Buffers
e. Equilibration
f. Column Purge Volumes
III. CaRe aND Use
a. Sample Preparation and Filtration
b. Flow Rate Range
c. Precautions
d. Storage Considerations
Iv. TesT CONDITIONs
a. Column Efficency
v. TROUbleshOOTINg
PROTeIN-Pak PW ION-exChaNge COlUmNs