Waters ACQUITY UPLC SEC Columns and Standards - Manual

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[ CARE AND USE MANUAL ]
ACQUITY UPLC BEH SEC Columns
ACQUITY UPLC BEH SEC COLUMNS AND STANDARDS
CONTENTS
I. INTRODUCTION
II. CONFIGURING AN ACQUITY UPLC SYSTEM FOR USE
IN SEC PROTEIN SEPARATIONS
a. Calibrators
III. GETTING STARTED
a. eCord ™ Installation
b. Column Connectors
c. Column Installation
d. Column Equilibration
e. Useful Functional Tests for Benchmarking a New Column
IV. COLUMN SPECIFICATIONS AND USE
a. SEC Eluent and Needle Wash Preparation
b. Sample Preparation
c. Column Specification
V. TROUBLESHOOTING
VI. COLUMN CLEANING, REGENERATION AND STORAGE
a. Cleaning and Regeneration
b. Storage
VII. INTRODUCING eCord INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VIII. ORDERING INFORMATION
I. INTRODUCTION
Thank you for choosing a Waters ACQUITY UPLC ® BEH SEC guard,
column, and/or standard mix that are integral parts of Waters
ACQUITY UPLC SEC System Solution. The BEH125 SEC offering is
best suited for the analysis of peptides and proteins in the molecular
weight range from 1,000–80,000 Daltons that include insulin and
its aggregates. The BEH200 SEC column was designed to characterize
proteins ranging in molecular weight range from 10,000–450,000
Daltons that include monoclonal IgG monomers from aggregates,
while our BEH450 SEC is best suited for the analyses of proteins
and conjugates that range from 100,000–1.5 million Daltons
(Fig 1). All of these SEC chemistries are based on Waters Ethylene
Bridged Hybrid (BEH)-based particle technology and diol-bonded
surface that provide a stable chemistry with minimal non-desired
secondary interactions for proteins and peptides. The columns are
manufactured in a cGMP, ISO 9001 certified plant using ultra
pure reagents. Each batch of Protein Separation Technology SEC
packing material has been tested and qualified using a protein
test mixture (that is also available for purchase), and the results
are held to narrow specification ranges to ensure reproducible
performance. Every column is also individually tested for efficiency.
A Performance Test Chromatogram along with a Certification of
Analysis are available with each column.