Waters Cytochrome c Digestion Standard - Manual

Cytochrome c Digestion Standard
Cont entS
I. IntroductIon
II. IdentIfyIng Parts
III. usIng the Waters
extractIon ManIfold
IV. needle tIP InstallatIon Procedure
V. sPare Parts and accessorIes
I. IntroductIon
The Cytochrome c Digestion Standard was prepared by digesting
Bovine Heart Cytochrome c (Uniprot, P/N P62894) with sequencing
grade trypsin. Each batch of digestion standard is prepared under
strict quality control criteria confirmed on the included certificate
of analysis. Protein digestion standards may be used for a variety
of applications, including evaluation and benchmarking of HPLC
and UPLC
® Columns or evaluation of mass spectrometry and
chromatography instrumentation performance.
column
XBridge™ BEH300 C
18
XBridge BEH130 C
18
ACQUITY UPLC CSH™ and XSelect™
ACQUITY UPLC ® BEH300 C
18
ACQUITY UPLC BEH130 C
18
Atlantis ® dC
18
Symmetry300™ C
18
BioSuite™ PA-B
BioSuite PA-A
II. Storage and StabIlIty
The lyophilized powder is stable at room temperature until the expiration
date printed on the label. Once reconstituted in 0.055% trifluoroacetic
acid in water, the solution is stable for one week when stored at 8 °C.
Stability can be extended by freezing samples at -20 °C.
III. reconStItutIon of the cytochrome c
dIgeStIon Standard for hPlc/uPlc
The protein digestion standards were prepared by digesting 1 nmol of
protein. For HPLC column evaluation, the recommended sample dilution
is 200 µl of 0.055% trifluoroacetic acid in water.
Table 1. Compatible chemistries that can be used with this standard.
[ Care and Use ManUal ]