Waters MassPREP Protein Standard Mix - Manual

[ CARE AND USE MANUAL ]
MassPREP Protein Standard Mix 1
I. INTRODUCTION
The MassPREP ™ Protein Standard Mix is a mixture of a variety of carefully
chosen proteins encompassing a wide range of properties. This mixture
contains proteins that vary in isoelectric points, molecular weights and
hydrophobicities. These characteristics provide the user with an attractive
intact protein mixture that can be used for a variety of applications.
Table 1 describes the composition of this mixture.
The standard was developed to provide a means to test Waters UPLC ®
and HPLC Protein Separation Columns such as the ACQUITY UPLC ®
BEH C
4
1.7 μm and XBridge ™ BEH300, C
4
3.5 µm column offerings.
In our quest to offer useful analytical tools, we now offer the same
protein standard mix used at Waters to QC batch test of some of
our Protein Separation Technology Column offerings. The standard is
prepared under strict quality control criteria confirmed on the specific
certificate of analysis that is available upon request for each batch
of prepared MassPREP Protein standard. In addition to evaluation of
HPLC/UPLC columns, this mixture may also be used for a variety of
other applications, including evaluation of mass spectrometry and
chromatography instrumentation performance.
Table 1. Composition of MassPREP Protein Standard Mix
Protein Sample
Molecular
Weight, MW
SwissProt
Sequence
Information
Part
Number
(Sigma)
Ribonuclease A, bovine pancreas 13.700 K P61823* R5500
Cytochrome c, horse heart, 96% 12.384 K P00004* C7752
Albumin, bovine serum, 96-99% 66.430 K P02769* A8022
Myoglobin, horse heart >90%
16.950 K plus
the heme group
616 K
Total:17.600 K
P68082 M1882
Enolase, from baker’s yeast
(S. cerevisiae)
46.671 K P00924 E6126
Phosphorylase b, rabbit muscle 97,200 K P00489 P6635
*Best match from database for more information on sequence please
reference the raw material vendor information (Sigma)
MASSPREP PROTEIN STANDARD MIX
CONTENTS
I. INTRODUCTION
II. STORAGE AND STABILITY
III. RECONSTITUTION OF MASSPREP PROTEIN STANDARD MIX
FOR HPLC/UPLC USE