Waters Viridis SFC Columns - Manual

[ Care and Use ManUal ]
1 Viridis SFC Columns
Contents
I. IntRoDUCtIon
II. GettInG stARteD
a. Safety Considerations
b. Column Installation
c. Column Equilibration
d. Initial Column Efficiency Determination
III. CoLUMn Use
a. Sample Preparation
b. Solvents
c. Pressure
IV. sCALInG UP/DoWn IsoCRAtIC MetHoDs
V. ConneCtInG tHe CoLUMn to tHe sFC sYsteM
a. Column Connectors and System Tubing Considerations
b. Band Spreading Minimization
VI. tRoUBLesHootInG
VII. CoLUMn CLeAnInG, ReGeneRAtInG AnD stoRAGe
a. Cleaning and Regeneration
b. Storage
I. IntRoDUCtIon
Thank you for choosing a Viridis ® SFC column. The Viridis SFC packing
materials are designed to provide excellent stability, minimal column
bleed, and high mass loading. The Viridis SFC packing materials are
manufactured in an ISO 9000 certified plant using ultra-pure reagents.
Each batch of Viridis SFC material is tested and the results are held to
narrow specification ranges to assure excellent, reproducible performance.
Every column is individually tested and a Performance Chromatogram is
provided with each column along with the Certificate of Batch Analysis.
Table 1. Physical Characteristics
Viridis
SFC
Chemistry
Particle
Shape
Particle
Size
(µm)
Pore
Size
(Å)
Surface
Area
(m 2 /g)
Pore
Volume
(cc/g)
Carbon
Load
(%)
End-
capped
2-Ethyl-
pyridine
Spherical 5 100 340 0.90 8 No
Silica Spherical 5 100 340 0.90 n/a n/a
VIRIDIs sFC CoLUMns