Waters Gen-Pak FAX Columns - Manual

[ Care and Use ManUal ]
Gen-Pak FAX Columns 1
Contents
I. IntRoDUCtIon
II. InstALLAtIon
III. GeneRAL UsAGe ConsIDeRAtIons
IV. GeneRAL MetHoD DeVeLoPMent GUIDeLInes
V. eXAMPLe UsInG HAe III DIGesteD DnA
VI. tRoUBLsHootInG
Waters Gen-Pak ™ FAX columns offer the highest resolution available in
anion- exchange HPLC of nucleicacids. The Gen-Pak FAX column
contains a weak anion exchanger based on DEAE functionalized non-porous
resin. It contains 2.5 µm particles and is well suited for analytical and
micro-preparative applications.
I. Int RoDUCtIon
This manual covers the use of the Waters Gen-Pak FAX column. The
Gen-Pak FAX column is a 4.6 x 100 mm steel column containing
a polymer-based high-performance anion-exchange packing. The
column is designed to perform high-resolution analysis and
purification on various nucleic acid species (up to at least
5,000 base pairs) such as DNA restriction fragments, polymerase chain
reaction (PCR) products, plasmids and synthetic oligonucleotides.
Gen-PAk fAX CoLUMns