Waters µStyragel Column - Manual

[ Care and Use ManUal ]
µStyragel Column 1
Contents
I. IntRoDUCtIon
a. Description
b. Characteristics of µStyragel™ Columns
II. InstallIng the ColUmn
a. Preparing the GPC/HPLC System
b. Connecting the Column to the GPC System
c. Connecting Columns in Series
d. Equilibrating the Column
III. PRePaRIng solVent anD samPles
a. Preparing the Solvent
b. Changing Solvents
c. Preparing the Sample
IV. UsIng the ColUmn
a. Chromatography Guidelines
b. Calibrating the Column
V. CaRe anD maIntenanCe
a. Chromatography Guidelines
b. Calibrating the Column
c. Converting Columns for High Temperature Chromatography
VI. oRDeRIng InFoRmatIon
µstyRagel ColUmn
I. IntRoDUCtIon
This manual covers the use of the Waters µStyragel TM family of Gel
Permeation Chromatography (GPC) columns. Please take a few moments to
read this carefully. The recommendations in this manual will help you obtain
good column life and reproducible chromatography.
a. Description
µStyragel columns are packed with high performance, fully porous,
highly cross-linked styrene divinylbenzene copolymer particles. This
column family offers you a choice in moderate to high efficiency, high
resolution, and stable, reproducible operation. Their different resolu-
tion and stability characteristics allow you to choose the optimum
column for your application.
b. Characteristics of µStyragel Columns
Table 1 lists characteristics of the different types of µStyragel
columns.
Table 1: Characteristics of µStyragel Columns
II. InstallIng the ColUmn
a. Preparing the GPC/HPLC System
Before attaching the column in the flow path on a GPC/HPLC system,
you must first prepare the system:
1. Directly connect the system injector to the detector by replacing
the old column(s) with a zero dead volume union.
2. Flush the system to remove any micro particulates and old
solvents. Flush the injector loop if applicable.
3. Remove the union.
Column
Type
Nominal
Particle Size
Shipped in Avoid using
µStyragel 15 µm Toluene Water, alcohols except
HFIP, ketones, or alkanes