Care and use manual – Waters Styragel Columns User Manual
Page 10
[ Care and Use ManUal ]
Styragel Columns
10
Table 6. Conditions and sample for 7.8 mm for Column Efficiency Test:
HT Columns1
Table 7. Conditions and sample for 4.6 mm for Column Efficiency Test:
HT Columns1
Figure 6. Tangent Method for Calculating Column Efficiency
N = 16 (tR/w)
2
tR = Elution distance from injection point to the peak
apex on the chart paper.
W= Peak width of marker measured where tangents
drawn on peak intersect baseline (same units as tRj.
Column Type
Toluene
THF
DMF
Flow Rate
(mL/min)
HT 3
ODCB
Acetone
Acetone
2.0
HT 4
ODCB
Acetone
Acetone
2.0
HT 5
ODCB
Acetone
Acetone
2.0
HT 6
DCHP
Acetone
Acetone
2.0
HT 6E
DCHP
Acetone
Acetone
2.0
Column
Type
Toluene
THF
DMF
Marker
Flow Rate
(mL/min)
Marker
Flow Rate
(mL/min)
Marker
Flow Rate
(mL/min)
HR 3
ODCB
0.3
Acetone
0.3
Acetone
0.3
HR 4
ODCB
0.3
Acetone
0.3
Acetone
0.3
HR 5
ODCB
0.3
Acetone
0.3
Acetone
0.3
HR 6
DCHP
0.3
Acetone
0.3
Acetone
0.3
HR 6E
DCHP
0.3
Acetone
0.3
Acetone
0.3
Table 8. Test Condition and Sample for Efficiency Test 7.8 mm HMW
Columns
Column Type
Toluene
THF
DMF
Flow Rate
(mL/min)
HMW 7
DCHP
Acetone
Acetone
2.0
HMW 6E
DCHP
Acetone
Acetone
2.0