Waters Oligonucleotide Separation Technology XBridge OST C18 Columns - Manual

[ method guidelines ]
XBridge ™ OST C
18
columns are based on Waters second generation of
hybrid-silica BEH Technology ™ particles and can be effectively used
for the lab scale purification and analysis of detritylated synthetic
oligonucleotides using ion-pair, reversed-phase chromatography. This
document provides useful method guidelines for the effective use of
this column chemistry for this group of compounds.
Contents
I. PRINCIPLES OF OLIGONUCLEOTIDE SEPARATIONS
II. SAMPLE PREPARATION
III. RECOMMENDED MOBILE PHASES
IV. RECOMMENDED INJECTOR WASH SOLVENT
V. GENERAL CONSIDERATIONS IN DEVELOPING SEPARATIONS
VI. ANALYSIS OF MODIFIED OLIGONUCLEOTIDES
VII. PURIFICATION CONSIDERATIONS
XBridge ™ ost C
18
Method guidelines
*ost: oligonuCleotide separation teChnology