Waters Oligonucleotide Separation Technology ACQUITY UPLC C18 Column - Manual

[ method guidelines ]
ACQUITY UPLC ® OST C
18
columns are based on Waters second gen-
eration of hybrid-silica BEH Technology™ particles and can be effec-
tively used for the high resolution analyses of detritylated synthetic
oligonucleotides using ion-pair, reversed-phase chromatography. This
document provides useful method guidelines for the effective use of
this column chemistry for this group of compounds.
Contents
I. PRINCIPLES OF OLIGONUCLEOTIDE SEPARATIONS
II. SAMPLE PREPARATION
III. RECOMMENDED MOBILE PHASES
IV. RECOMMENDED INJECTOR WASH SOLVENTS
V. SOLVENT MIXING OPTIONS
VI. DEVELOPING SEPARATIONS
VII. ANALYSIS OF MODIFIED OLIGONUCLEOTIDES
ACQUIt Y UPLC ® ost C
18
*ost: oLIgonUCLeotIde sePArAtIon teChnoLogY