Waters Oligonucleotide Separation Technology ACQUITY UPLC C18 Columns - Manual (Page 6)

[ Care and Use ManUal ]
6
b. Installation
Install the column into the column heater. Plug the eCord™ into the
side of the column heater. Once the eCord™ is inserted into the col-
umn heater the identification and overall column usage information
will be available in Empower
® and MassLynx ® software allowing the
user to access column information on their desktop.
c. manufacturing Information
The eCord™ chip provides the user with an overview of the bulk
material QC test results.
The eCord™ chip provides the user with QC test conditions and results on
the column run by the manufacturer. The information includes mobile
phases, running conditions and analytes used to test the columns. In
addition the QC results and acceptance is placed onto the column.
d. Customer use Information
The eCord™ chip will automatically capture column use data. The top
of the screen identifies the column including chemistry type, column
dimensions and serial number. The overall column usage information
includes the total number of samples, total number of injections,
total sample sets, date of first injection, date of last injection,
maximum pressure and temperature. The information also details
the column history by sample set including date started, sample set
name, user name, system name, number of injections in the sample
set, number of samples in the sample set, maximum pressure and
temperature in the sample set and if the column met basic system
suitability requirements.
V. addItIonal InformatIon
a. Band Spreading minimization
Waters ACQUITY UPLC
® System is designed to have a minimal post
column band spreading. If desirable, the mass spectrometer can be
connected either directly or in series with UV (PDA) detector. The con-
necting tubing internal diameter should be 100 µm or less in order
to preserve the achieved separation. Length of the tubing should also
be kept to a minimum.
Detritylated synthetic oligonucleotide separations are almost
exclusively performed using gradient elution techniques. As such,
the effect of pre-column sample band broadening can be minimized
by allowing the sample to bind to the column before beginning the
actual separation gradient. However, proper connection from the
ACQUITY UPLC
® OST C
18
column outlet to the detector is critical in
order to minimize the deleterious effect of post-column sample band
spreading. Use of appropriate internal diameter tubing (e.g., 0.005
inch PEEK™ tubing for UV detector applications) is recommended.