LaMotte MF 3-0035 - Manual (Page 3)

150mL
100
150mL
100
50
A - Apparatus Lid
B - Funnel
D - Filter Holder with O ring
F - Collection Container
I - Barrel
H - Plunger
J - Tubing
E - (Membrane Filter)
C - (White Pad)
G - Adapter
Pro ce dure
1. Thaw a bottle of Coliscan MF overnight at room temperature. (2 mL of Coliscan
MF is required for each sample to be tested). Bottles may also be thawed for
same day use by standing the bottles in warm water until the contents become
liquid.
Sample Collection and Dilution
2. Collect the water sample according to the chart below.
Environmental river, lake, pond, stream, ditch 1.0 to 5.0 mL
Drinking water well, municipal, bottled 100 mL
Generally, use 100 mL for samples suspected of having very low coliform levels such
as drinking water. Use 1.0 to 5.0 mL for samples suspected of having moderate
amounts of coliform, such as environmental samples (rivers, ponds, streams, lakes or
ditches). Samples that are less than 10 mL will need to be diluted with 10 – 20 mL of
a sterile diluent before filtering.
For optimum results, the sample should be collected within two hours before filtering.
If this is not possible, the sample may be refrigerated or kept on ice for up to 24
hours.
3. Use a permanent marker or wax pencil to
label a dish with pad with the sample information.
4. Use the sterile 3 mL dropper to add 2mL of
Coliscan MF to the pad in the dish. Cover the
dish with a lid.
Set up filter apparatus
5. Remove the sterile filter apparatus from the packaging.
Smaller diameter
Contains Clear
Media Film
Lid
Bottom
3