Confidence level, Manually changing clusters – Bio-Rad Precision Melt Analysis™ Software User Manual
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Precision Melt Analysis Software Instruction Manual
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Confidence Level
Precision Melt Analysis software determines a probability distribution for each cluster based
on the standard deviation of the melt curves within the same cluster. Each sample is mapped
onto each cluster's probability distribution, based on that sample's similarity to the mean melt
curve across each sample in the cluster. The confidence value is an indication of the relative
probability the sample has of being in a cluster.
Manually Changing Clusters
In the Precision Melt tab spreadsheet, select options from the pull-down menu in a cell in the
the Cluster column to override the cluster assignment or change cluster properties (Figure 31).
Figure 31. Changing clusters in the spreadsheet.
Options to change the cluster assignment include the following:
•
Select from the list of cluster names to assign the sample well(s) to a new cluster
•
Select <Create New Cluster> to assign the selected well to a new cluster. Enter the
name of the new cluster in the Name window and click OK
•
If you previously changed the automatic call of a sample, select <Undo Manual
Cluster> to change the cluster assignment back to the software called cluster
•
Select <Exclude from Clusters> to remove the well from the assigned cluster. Wells
excluded from clusters display Excluded in the Cluster column in the data
spreadsheet. Select <Include in Clusters> to re-include the sample in the cluster
analysis
Changes made to the cluster are applied to all samples in the cluster. Options to change the
cluster parameters include the following:
•
Select <Set Cluster Name> to assign a name to the cluster. Enter the name of the
cluster in the Rename window text box and click OK
•
Select <Set Cluster Color> to change the color of the fluorescence traces for the
wells assigned to the selected cluster. Choose the new color from the color options
in the Color window and click OK
•
Select <Set as the Reference Cluster> to use the selected cluster as the Difference
Curve reference cluster.
NOTE: The above mentioned changes can also be done on the chart.