Bio-Rad Gene Pulser® Electroporation Buffer - Manual

Gene Pulser ® Electroporation Buffer Reagent
Catalog # Volume
165-2676 10 x 1.8 ml
165-2677 30.0 ml
For research only. Store at 4°C.
Storage and Stability
Gene Pulser electroporation buffer reagent is shipped on ice. Store at 4°C upon receipt. Gene Pulser electroporation
buffer is stable for 6 months from date of purchase when stored at 4°C
.
Handling Precautions
Gene Pulser electroporation buffer reagent is intended for the delivery of siRNAs and plasmid DNA into cultured
mammalian cells. It is nuclease-free to prevent the degradation of siRNAs or plasmid DNA. Use gloves and nuclease-free
reagents, pipets, and tubes, as well as good tissue culture practices.
Contents
Electroporation buffer contains 10 x 1.8 ml (catalog #165-2676) or 30.0 ml (catalog #165-2677) of a proprietary buffer
formulation. 1 ml is generally sufficient for 5–10 transfections in 0.2 cm cuvettes, 1–2 transfections in 0.4 cm cuvettes,
or 5–10 transfections in a 96-well plate.
Note: One 30 ml bottle is sufficient for electroporating two entire plates (96-, 24-, or 12-well plates). You do not have to
fill all wells in each plate
Overview
Electroporation is the preferred method for introducing biomolecules into cells, especially into cells that are resistant
to other methods of transfection. However, this method requires an effective electroporation buffer and optimized
conditions to ensure high cell viability after electroporation.
Gene Pulser electroporation buffer reagent was developed for the transfection of siRNAs and plasmid DNA into a
variety of cell types, resulting in high efficiency and low cytotoxicity.
Gene Pulser electroporation buffer reagent allows transfection using a variety of protocols and conditions, can be
used with any multiwell plate format or cuvette size, and can be used in Gene Pulser
® II, Gene Pulser Xcell ™ , Gene
Pulser MXcell ™ , and other electroporation systems.
For best results, it is important to determine the optimal amount of siRNA or plasmid DNA, and the
optimal electroporation conditions for any given cell line.
Recommendations for Optimal Results
Gene Pulser electroporation buffer reagent has been developed to achieve consistent transfection efficiencies using a broad
range of cell types with an easy-to-use protocol.
Optimum transfection efficiencies are achieved by adjusting:
• siRNA or plasmid DNA concentration
• Cell density at the time of transfection
• Waveform (exponential decay or square waveforms)
• Voltage
• Pulse
length
Once maximum transfection efficiency has been established, the conditions should be kept constant between experiments for
any particular cell line.
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