Bio-Rad ProteoMiner Protein Enrichment Kits User Manual

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Wash Buffer. 50 ml PBS buffer (150 mM NaCl, 10 mM NaH

2

PO

4

, pH 7.4)

Elution Reagent. 5 vials, lyophilized urea CHAPS (8 M urea, 2% CHAPS)

Rehydration Reagent. 5 ml, 5% acetic acid

Collection Tubes. 20 capless, 2 ml centrifuge tubes; 10 capped, 2 ml
centrifuge tubes

• Instruction

Manual

Quick Start Guide

ProteoMiner Introductory Small-Capacity Kit (Catalog #163-3008)

Contains two spin columns, all other reagents are the same as in the
ProteoMiner small-capacity kit above. Introductory kit provides reagents for
processing two samples.

ProteoMiner Introductory Large-Capacity Kit (Catalog #163-3009)

Contains two spin columns, all other reagents are the same as in the
ProteoMiner large-capacity kit above. Introductory kit provides reagents for
processing two samples.

ProteoMiner Sequential Elution Small-Capacity Kit (Catalog

#163-3010)

This kit combines the ProteoMiner small-capacity kit (catalog #163-3006) and
the ProteoMiner sequential elution reagents (catalog #163-3003) and is
designed to provide multiple elution options for researchers using SELDI or
other downstream protein separation analysis methods, and who wish to
access additional proteins. This kit is NOT compatible with 2-D gel
electrophoresis.

ProteoMiner Sequential Elution Large-Capacity Kit (Catalog

#163-3011)

` This kit combines the ProteoMiner large-capacity kit (catalog #163-3007) and

the ProteoMiner sequential elution reagents (catalog #163-3003) and is
designed to provide multiple elution options for researchers using SELDI or
other downstream protein separation analysis methods, and who wish to
access additional proteins. This kit is NOT compatible with 2-D gel
electrophoresis.

ProteoMiner Sequential Elution Reagents (Catalog #163-3003)

To be used in combination with the ProteoMiner kits (catalog #s163-3006 or
163-3007), this product provides reagents for processing 10 samples. The
sequential elution reagents are available for researchers using SELDI or other
downstream protein separation analysis methods, and who wish to access

2

10010636D:4110137B-4.0.GR.qxd

3/12/2009

7:51 AM

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