Bio-Rad Apoptosis Assays - Manual

This guide can be used to prepare and run a full 1 x 96-well assay plate.
For more information on a given step, refer to the corresponding section
of the complete instruction manual. New users can download the manual,
which includes detailed instructions and a list of kit components, at
www.bio-rad.com/bio-plex.
A. Reagent Preparation
1. Reconstitute the following lyophilized reagents in dH
2
0 before use
according to the table below.
a. Allow vial to sit at room temperature for a minimum of 5 min, not to
exceed 30 min.
b. Mix by vortexing at a medium setting.
2. Bring the 10x assay buffer to ambient temperature (RT).
a. Mix by inversion to ensure all salts are into solution.
b. Prepare 1x assay buffer — dilute 1 part 10x assay buffer (60 ml) with
9 parts of dH
2
0 (540 ml).
Bio-Plex Pro ™ RBM Apoptosis Assays
Quick Guide
IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.
Deviation from the protocol may result in low assay signal and assay variability.
For Use With
Instruction Manual #
Bio-Plex Pro ™ RBM Apoptosis Assays 10033631
Reagent Volume, µl Reagent Volume, ml
Standards mix 150 Blocking buffer 1.5
Control 1 100 Standard diluent 1.0
Control 2 100 Detection antibodies 4.8