Bio-Rad ChromLab™ Software - Manual (Page 43)

Chromatogram View
User Guide | 41
Changing the X-Axis Units and Scale
For runs performed from saved methods, the x-axis unit can be changed to Time
(min), Volume (ml), or Column Volume (CV). For runs performed manually, the x-axis
unit can be changed to Time (min) or Volume (ml).
To change the x-axis units and scale
 Click the x-axis title to toggle among the available options.
Changing the Y-Scale Values
You can change the maximum and minimum y-scale values in the legend to set the
chromatogram scale.
To change the y-scale values
1. Expand the legend in the chromatogram view.
2. Enter maximum and minimum values in the appropriate trace row, ensuring that
the maximum value always exceeds the minimum value.
Tip: See Trace Settings Tab on page 74 to revert the y-scale values to their
factory default settings.
Autoscaling the UV Trace
Autoscaling the UV trace scales the chromatogram’s primary UV y-axis based on
signal intensity. Autoscaling is enabled by default.
When multiple UV absorbance traces are present you can
 Autoscale each UV trace independently .
The y-axis scale of each trace is scaled to the UV signal intensity of the
individual trace.
 Autoscale all UV traces to the same scale .
The y-axis scale is based on the signal intensity of the UV trace with the highest
intensity and all other traces are normalized to the intensity of that trace.
 Disable autoscaling .