Bio-Rad BioLogic DuoFlow Software - Manual (Page 3)

intermolecular interactions. A purification protocol that is satisfactory for one molecule
may not work for another molecule. Several factors influence the quality of a purification
procedure. Some of these factors include: buffer composition (pH, ionic strength,
co-solutes), elution type (gradient slope and gradient duration), flow rate, column chemistry
and sample composition. In principle, each of these can be adjusted to produce the most
efficient and effective purification procedure for a molecule. However, in practice, only a
few of these are generally tested due to time and cost considerations. By performing
Scouting as a set of automated runs, the time and resources required for protocol
optimization can be significantly reduced.
Buffer Editor
The Buffer Editor is used to create new Buffer Blending buffer systems and to modify existing
buffer systems. Buffer Blending is a feature of the BioLogic DuoFlow Maximizer
™ and
BioLogic DuoFlow Pathfinder ™ systems that dynamically "blends" the conjugate acid and
base of a buffer with water and salt to produce a solution with a specific salt concentration
and pH. The Buffer Editor can be used to create new Buffer Blending buffer systems from
user-supplied information. Each buffer system can include up to three buffers and each buffer
can have up to three pKa's. The Buffer Editor also generates recipe text that describes how
to make each Buffer Blending solution. The Buffer Editor requires information about the buffer
( pKa(s), temperature coefficient(s), charge state, molecular weight and concentration) and
the salt (charge state molecular weight and concentration). This information can also be
supplemented with user determined 1 or 2 point pH corrections (entered in the Buffer
Blending setup dialog). For multiple component buffers, pH corrections can be included for
each buffer in the buffer system, to improve the pH accuracy over the entire buffer pH range.
The Buffer Editor can also be used to copy and modify any of the 26 predefined Buffer
Blending buffer systems. Each of these buffer systems can be modified to accommodate
reagents that are readily available in your laboratory.