Bio-Rad BioLogic QuadTec™ Detector and Components - Manual (Page 40)

The number of the scan to be performed or given out is selected by moving the cursor to the scan number,
(e.g. no.:1 with the Right arrow.) The scan number can be changed using the Up/Down arrows. The
wavelength ranges for all scans can be varied between three options:
190 to 380 nm
381 to 740 nm
190 to 740 nm
The wavelength range is selected by moving the cursor with the Right arrow to the corresponding field and
by choosing the appropriate wavelength range by toggling the Up/Down arrows. The selected range applies
always for all four scan numbers.
Caution: If the wavelength range is changed, spectral data in the memory of the QuadTec detector are
erased.
4.5.2 Scan Autozero
Before scanning spectra, the QuadTec detector must perform a scan autozero. The scan autozero measures
the spectrum of the solvent. These data are stored for comparison of the spectra of your analytes with the
spectrum of your solvent. Thus, a new scan autozero should be performed each time the solvent is changed.
A scan autozero is performed by pressing the green Auto Zero button for 2 seconds or longer. The autozero
is then measured and calculated over the wavelength range set for scanning.
4.5.3 Performing Wavelength Scans
To acquire scan data:
1. Enter the SCAN menu by pressing the yellow SCAN button once.
2. When pressing the SCAN button a second time, a scan is performed immediately.
3. When taking different scans during a run, be sure to adjust the scan number between scans. Stored
scans are overwritten without any message, when the SCAN button is pressed twice.
4.5.4 Output of Scan Data
The stored scan data can be given out via analog outputs and with defined data rates.
Note that the scan analog output is fixed at 10 AU = 1 V and is not changeable by the user. Use your chart
recorder voltage selection range to scale the output as needed.
Time scaling of the data can be adjusted between 1 and 50 steps per second (approximately 1–50 nm/s) in
the SETUP menu. Low output speed settings lead to enhanced spectral resolution of the data output.
To output scanned data,
1. Move the cursor with the Right arrow to the OUT field in the scan menu.
2. Press the Up/Down arrows to start the scan output. Make sure that the desired scan number is
chosen. Since every scan is kept in internal storage it can be edited or given out several times.
Scans are deleted by overwriting with new scan data or when the wavelength range is changed.
USE AS A STAND-ALONE SYSTEM SYSTEM INSTALLATION AND SETUP
4-13