Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane II) - Manual (Page 15)

Additional ReadyPrep 2-D rehydration/sample buffer 1
is available and sold separately for this purpose (see
Section 8).
Note: For some applications (for example using basic pH
range IPG strips), the membrane protein sample may
require reduction and alkylation treatment before IEF in
order to achieve optimal 2-D separation. The ReadyPrep
Reduction-Alkylation Kit can be purchased separately for
this purpose (catalog #163-2090).
18. The samples are now ready to be loaded onto IPG
strips. Unused membrane protein extracts should be
stored in aliquots at –80°C.
Note when working with plant leaf samples: for best 2-D
separation results treat the sample using the ReadyPrep
2-D Cleanup Kit (Bio-Rad catalog #163-2130) prior to
performing IEF.
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