Bio-Rad ReadyPrep Protein Extraction Kit (Signal) User Manual
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Section 1
Introduction
The ReadyPrep protein extraction kit (signal) is designed as
a simple, rapid and reproducible method to prepare a
cellular protein fraction that is enriched in membrane-
associated signaling proteins. This kit is part of a group of
related kits from Bio-Rad developed to reduce sample
complexity in order to improve the chances of identifying
low-abundance and particular classes of proteins, such as
signaling proteins, and to simplify proteomic studies. The
fractionation protocol can be applied to a wide variety of
biological samples and the isolated proteins are suitable for
2-D gel analysis or other applications such as SDS-PAGE
and western blotting.
The plasma membrane of most cell types contains
organized microdomain structures that are rich in
sphingolipids and cholesterol and have distinct protein
compositions. Proteins associated with these lipid rafts are
involved in intracellular membrane trafficking and signaling
pathways, and include glycosylphosphatidylinositol (GPI)-
anchored proteins, caveolin, caveolae(caveolin)-associated
proteins, acylated tyrosine kinases, heterotrimeric
G-proteins and some proteins with transmembrane
domains (Simons and Ikonen 1997, Brown and Rose 1992,
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