Bio-Rad Microbial Culturing Module User Manual

Page 3

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Table of Contents

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Introduction........................................................................................................................i

Kit Inventory Checklist ......................................................................................................1

Safety Issues....................................................................................................................1

Preparation of Liquid Media ..............................................................................................2

Preparation of Ampicillin ............................................................................................2

Preparation of LB Broth..............................................................................................2

Preparation of Ampicillin Broth ..................................................................................3

Starting Minipreps ......................................................................................................3

Preparation of Solid Media ..............................................................................................4

Preparation of Ampicillin ............................................................................................4

Preparation of LB Agar ..............................................................................................4

Preparation of LB-Ampicillin Agar ..............................................................................5

Preparation of LB-Ampicillin IPTG Agar ....................................................................5

Procedure for Pouring Plates............................................................................................5

Streaking Plates with Bacteria ..........................................................................................6

Glossary ..........................................................................................................................7

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