Hudson Robotics Dried Blood Spot Processor - Manual (Page 11)

DBS Processor Page 9
1. Factory Calibration Method
Step 1:
Properly connect all cables and power-up the machine as described in
Sections III.A.1 and III.A.2, respectively.
Step 2:
From the Main Screen, enter the Calibration Screen by selecting
“Calibrate”. This function is password protected.
Step 3:
Press the Home button to home the XY stage.
Step 4:
The operator should prompt the XY stage to move to the load position.
Step 5:
Load a micro-titer plate.
Step 6: The operator should prompt the XY Stage to move to well A1.
Step 7:
Remove the punch mechanism front panel.
Step 8:
The operator can slide open the clear lexan door so as to have a clear view
of the micro-titer plate.
Step 9:
Loosen the two punch pin clamp screws. The punch is now free to move
freely in and out of the microtiter wells. Caution must be exercised not to move the
table with the punch down. Note: a small piece of thin cardboard can be used to hold
the punch up.
Step 10: If the punch tool enters the well without interference, then the operator
should set this location by clicking the Save Point button.
Step 10a: If the punch tool does not enter the well without interference, and/or if the
XY stage is not in the proper position under the punch tool, then the operator should
reposition the XY stage so that the punch tool enters the well without interference.
The operator can reposition the XY stage using the Jog Interval and Jog Arrows.
Use the Jog Interval to set the magnitude of the jog amount (distance you wish to
move with each jog). Use the Jog Arrows to jog the XY stage in either plus or minus
X or Y direction until the XY stage is properly positioned with the calibration well
directly under the punch. Note: the XY stage will move when a jog arrow is
activated. Once the XY stage is in the proper position, set this location as described
in Step 10 above. Note: The XY Position Display may be helpful to the operator to
determine the approximate magnitude of the jog interval required for the move.
Step 11: The operator should repeat this procedure for wells A12, H1, and H12.
Step 12: When all four (4) calibration points are set. The operator should press the
Calibrate Plate button. The operator will be prompted to recalculate all well