ChemoMetec NC-200 - Manual (Page 6)

5
Optional: Visual Inspection
of Counting Gates
OPTION
(PREFERRED)
Click the green dot in the right-hand corner of the image window.
This activates the image overlay function indicating the events includ-
ed in the total cell count. The mouse scroll button allows the user to
zoom in at the cursor position. Cells will be framed by a pink square.
OPTION
(ADVANCED)
Right-click on the sample file name in the ’Data folders and
files’ window and select ’Show counting gates in Plot Man-
ager’.
A new window will open displaying scatter plots and histograms of
event intensity and size for the appropriate channels (AO and DAPI).
It is important that the core of the population, usually seen as a col-
oured region on the scatter plot, is included in the square counting
gate.
Note it is not required that all events be included in the counting
gate. Events in the lower, left-hand corner of the scatter plot are often
smaller than cells and considered debris.